Kadar mRNA Hypoxia Inducible Factor Alpha Plasma Darah Pasien Kanker Ovarium Tipe Mucinous Dan Serous

Siti Nur Chasanah, Teguh Aryandono, Sofia Mubarika Haryana

Abstract


Proses karsinogenesis pada kanker ovarium melibatkan regulasi tingkat molekular, salah satunya adalah mRNA. Beberapa penelitian sebelumnya menyebutkan bahwa disregulasi mRNA HIF1A terjadi pada berbagai kanker. Penelitian melaporkan adanya peningkatan ekspresi mRNA HIF1A pada kanker ovarium. Kanker sering berada dalam kondisi hipoksia, mRNA HIF1A merupakan regulator gen pada kondisi hipoksia. HIF1A terlibat dalam berbagai hallmark kanker. Berdasarkan hal tersebut, penelitian ini dilakukan untuk mengetahui apakah terdapat perbedaan ekspresi mRNA HIF1A pada plasma penderita kanker ovarium tipe mucinous dan serous. Sampel plasma diambil dari pasien kanker ovarium di RSUP Dr. Sardjito, tipe mucinous sebanyak 9 sampel dan tipe serous sebanyak 9 sampel. Total RNA diisolasi dari sampel plasma darah pasien kanker ovarium dengan menggunakan miRCURY RNA isolation Kit-Biofluid. Kuantifikasi mRNA HIF1A dilakukan One-step qRT-PCR dengan kit KAPA TM SYBR®. Hasil qRT-PCR dianalisis dengan menggunakan Biorad CFX Manager TM Software. Hasil analisis menunjukkan bahwa terdapat perbedaan ekspresi mRNA HIF1A pada tipe serous dibandingkan dengan tipe mucinous (p value =0,018). Ekspresi mRNA HIF1A pada tipe serous meningkat 5,62 kali lipat dibandingkan dengan tipe mucinous. Penelitian ini telah berhasil membuktikan bahwa mRNA HIF1A lebih tinggi pada kanker ovarium tipe serous. 


Keywords


Plasma, mRNA HIF1A, Serous, Mucinous

DOI:

https://doi.org/10.30596/jph.v1i2.4613


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