UJI EFEKTIVITAS ANTIBIOTIK EKSTRAK DAUN CENGKEH (SYZYGIUM AROMATICUM) TERHADAP PERTUMBUHAN BAKTERI SALMONELLA TYPI SECARA IN VITRO

Dilla Ulfa Ristiansyah, Yenita Yenita, Melviana Melviana, Annisa Annisa

Abstract


Typhoid fever is an enteric bacterial infection caused by Salmonella enterica serovar Typhi or Paratyphi. Salmonella typhi is a Gram negative rod which has no spores and moves with flagel peritric. Salmonella is facultative intracellular and facultative anaerobes. Clove leaves (Syzygium aromaticum) have antibiotic effects on bacteria and known can inhibit Salmonella typhi bacteria. Methodology: This study used an experimental method. The technique used in measuring antibiotic activity is the method of disk diffusion. Result: The concentration of 10%, 15%, 20% and 25% clove leaf extracts resulted in average of 11.89 mm, 11.40 mm, 16.76 mm, and 16.90 mm respectively. while the clear zone diameter of chloramphenicol is 20.74 mm.Conclusion: Clove leaf extract at 25% concentration has high clear zone in treatment group. Keywords: Salmonella typhi, Clove Leaf extract

Keywords : Salmonella typhi, Cengke leaf’s extract    



DOI:

https://doi.org/10.30596/isb.v2i1.1901


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References


Butler T. Treatment of Typhoid Fever in the 21st Century: Promises and Shortcomings, Department of Microbiology and Immunology, 2011 ; 17 (7), 959- 963.

Zhu, Q., Lim, C.K., Chan, Y.N. Detectionof Salmonella typhi by Polymerase Chain Reaction. Journal of Applied Bacteriology. 1996 ; 80:244-251.

Iswari,R.,Asmono,N., Santoso, U.S., S. Lina. Pola kepekaan kuman Salmonella terhadap obat kloramfenikol, ampisilin dan kotrimoksazol selama kurun waktu 1979- 24. 1983. Majalah Kedokteran Indonesia. 1998 ; 36:13- 19

Shulman, T.S., Phair, J.P dan Sommers, H.M. Dasar biologis dan klinis penyakit infeksi, Edisi ke-4 (terjemahan), Yogyakarta, Gadjah Mada University Press 2005 ; 300-305.

Departemen Kesehatan Republik Indonesia, 2013. Riset Kesehatan Dasar, Badan Penelitian dan Pengembangan Kesehatan Departemen Kesehatan, Jakarta.

Amelia R. Profil penderita demam tifoid pada orang dewasa di rsud dr. Pirngadi medan pada april 2012 – april 2013. Skripsi. 2013

Pratiwi GA. Uji daya hambat ekstrak daun cengkeh (Syzygium Aromaticum (L.) Merr & LM Perry) terhadap pertumbuhan Candida albicans [Skripsi]. Manado: Universitas Sam Ratulangi ; 2015

Kurniawan A, Rahayu S, Wahtuningrum R. Perbandingan kadar eugenol minyak atrisi daun cengkeh (Syzygium Aromaticum (L) Merry & Perry) yang tumbuh di dataran tinggi dan dataran rendah. Jurnal Pharmacy. 2009 ; 6(3).

Haryani D. Berkumur ekstrak daun cengkeh (Eugenia Aromaticum) 4% dapat menurunkan jumlah koloni bakteri dan bakteri Staphylococcus Aureus pada abses submukus. Denpasar: Universitas Udayana; 2015.

Tr iAtmodjo, P dan Triningsih,E.M. Besarnya kasus demam tifoid di Indonesia dan pola resisten Salmonellatyphi terhadap antibiotika. Majalah Kesehatan Masyarakat Indonesia. 1998 ; 5:261-263.

Klotchko, A., 2011. Salmonellosis. Available from: http://emedicine.medscape.com/article/228174-overview. [Accessed 10 juni 2017]

Su, L.H., Chiu, C.H., 2007. Clinical Importance and Evaluation of Nomenclature Available from: http://reference.medscape.com/medline/abstract/17760271 [ Accessed 11 juni 2017]

Levinson, W. Review of Medical Microbiology and Immunology ,10th edition. California: Mc Graw Hill: 2008 ; 133-142

Brooks, G.F., Butel, J.S., Morse, S.A., Jewetz, Melnick,& Adelberg Mikrobiologi Kedokteran, Edisi 23. Jakarta: Penerbit Buku Kedokteran ECG. 2004 ; 251-264

Aslam, A.B.N., 2010. Efektifitas protein Adh O36-Salmonella, Available from: elibrary.ub.ac.id./Efektivitas-protein-AdhO36-Salmonella [ Accessed 15 May 2017]

Dieye, Y., Ameiss, K., Mellata, M., Curtiss, R., 2009. Salmonella Pathogenecity Island SPI1 Contribute More Than SPI2 to The Colonization of The Chicken by Salmonellar enterica. Available from: http://www.biomedcentral.com/1471-2180/9/3. [Accessed 19 May 2017]

Parry, C.M., 2002. Typhoid Fever. Available from: http://www.nejm.org/doi/pdf/10.1056/NEJMra020201 [Accessed 19 mei 2017]

Murray, P.R., Rosenthal, K.S., and Pfaller, M.A., Medical Microbiology 6 edition. Canada : Mosby Elsevier 2009. 301-309

Bulan, R. 2004. Reaksi Asetilasi Eugenol dan Oksidasi Metil Iso Eugenol. http://www.google.co.id/search?hl=id&q=reaksi+asetilasi+eugenol+dan+oksidasi+metil+eugenol&meta=&aq=f&oq . Diakses tanggal 3 Juli 2017.

Sharma, S.K., V.K. Srivastava and R.V. Jasra. Selective double bond isomerization of allyl phenylmethers catalyzed by ruthenium metal complexes. Journal of Molecular Catalysis A : Chemical . 2006.245 : 200- 209.

Jirovetz, L. Medicinal value of clove. University of Vienna, Departement Pharmacy and Diagnostics, Austria. http://herbication.com . 2010 . diunduh pada [ 16 juni 2017].

Naiborhu PE. Ekstraksi dan manfaat ekstrak mangrove (Sonneratia alba dan Sonneratia caseolaris) sebagai bahan alami antibakterial: Pada Patogen Udang Windu, Vibrio harveyi. 2002

INDON P. Kandungan fitokimia beberapa jenis tumbuhan lokal yang sering dimanfaatkan sebagai bahan baku obat di Pulau Lombok. 2015 Apr;1(2): 388-391.

Wayan FA, Betta K. Binahong (Cassia Alata L) As Inhibiting Of Escherichia Coli Growth. Lampung: Universitas Lampung. Vol. 4 No.4. 2008

Monalisa D, Handayani T dan Sukmawati. Uji daya hambat bakteri ekstrak daun tapak liman ( Elephentopus scaber L.) terhadap Staphylococcus aureus dan Salmonella typhi. Jurnal BIOMA. 9(2).13-20 . 2011.

Devi KP, Nisha SA, Saktivhel R, el all. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. PubMed. 2010. 130 (1) ; 170 -115

Katzung, B. G. Farmakologi Dasar dan Klinik Buku 3 Edisi 8. Penerjemah dan editor: Bagian Farmakologi FK UNAIR. Penerbit Salemba Medika, Surabaya. 2004 ;37- 41

Pratiwi Sylvia T. Mikrobiologi Farmasi Jakarta: Erlangga; 2008.

Syamsuni H.A. Ilmu Resep. Jakarta: EGC; 2006

Ditjen POM. Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta : Departemen Kesehatan RI; 2002. h. 1, 10-2

CLSI. Performance standart for antimicrobial susceptibility testing; twenty-first informational supplement. M100-S21. Vol. 31 No.1. Clinical and Laboratory Standards institute. USA.2011.

Andries JR, Gunawan PN, Supit A. Uji efek antibakteri ekstrak bunga cengkeh terhadap bakteri Streptococcus mutans secara in vitro. e-G. 2014;2.

Paliling A, Pisang J, Anindita P.S. Uji Daya Hambat Ekstrak Bunga Cengkeh (Syzygium aromaticum) Terhadap bakteri Porphyromonas Gingivalis. Manado: Jurnal e-GiGi. Vol. 4. 2016. Hal: 229-234

Kumala S, Indriani D. Uji Antibakteri Ekstrak Daun Cengkeh (syzygium aromaticum. Jakarta selatan: Jurnal Farmasi Indonesia. Vol. 4 No.2. 2008

Kaawon PT, Abidjulu J, Siangian KV. Uji daya hambat ekstrak buah pala (myristica fragnans houtt) terhadap bakteri penyebab peridonitis porphyromonas gingivalis secara in vitro. e-GIGI. 2016;4(2)


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