Delken Kuswanto, I Nyoman Ehrich Lister, Ermi Girsang, Ali Napiah Nasution, Wahyu Widowati


Free radicals in the body will increase with excessive of ultraviolet (UV) light exposure, induce oxidative stress with the formation of Reactive Oxygen Species (ROS). A visible effect on skin tissue known as photoaging, including the process of melanogenesis catalyzed by the tyrosinase enzyme, risked for pigmentation or melanoma disorder.

The use of natural ingredients has been widely used by the community to prevent the aging process. Natural compounds from a plant can be a source of antioxidants and have anti-aging abilities through inhibition of the tyrosinase enzyme. Black soybean (Glycine max (L.) Merr.) are high in isoflavone compound, one of which is daidzein.

This study evaluates the antioxidant and anti-aging potential of black soybean extract with daidzein. Antioxidants using the DPPH method and anti-aging tests carried out, namely the inhibition of the tyrosinase enzyme, are very important in the aging process.

Daidzein has an IC50 value of DPPH scavenging activity around 109.34±2.80 µg/mL lower than black soybean extract with 116.52±2.50 µg/mL. Results on tyrosinase enzyme inhibition activity, black soybean extract had an IC50 value of 70.71±1.83 µg/mL lower compared to daidzein with 72.65±2.81 µg/mL. In contrast to the better antioxidant activity of daidzein, the black soybean extract are more potential to inhibit the enzyme tyrosinase


Full Text:



Tobin DJ. Introduction to skin aging. Journal of tissue. 2016; 26(1):1-5.

Lai X, Wichers HJ, Soler-Lopez M, Dijkstra BW. Structure and Function of Human Tyrosinase and Tyrosinase-Related Proteins. Chem. Eur. J. 2018; 24:50-53.

Siti ZR, Norkhadijah SIS, Praveena SM. Hazardous Ingredients in Cosmetics and Personal Care Products and Health Concern: A Review. Public Health Research. 2015; 5(1):1.

World Health Organization. Traditional Medicine Strategy. 2013.

Iris FFB & Sissi WG. Herbal medicine : biomolecular and clinical aspects, 2nd Ed. New York: Taylor&Francis Group, (Chapter 1). 2011.

Arifin AS. Kajian Morfologi Anatomi dan Agronomi antara Kedelai Sehat dengan Kedelai Terserang Cowpea Mild Mottle Virus serta Pemanfaatannya sebagai Bahan Ajar Sekolah Menengah Kejuruan. Jurnal Pendidikan Sains. 2013; 1(2):115.

Alghamdi et al. Comparative phytochemical profiling of different soybean (Glycine max (L.) Merr) genotypes using GC–MS. Saudi Journal of Biological Sciences. 2018; 25:15.

Sumardi et al. Potential of local black soybean as a source of the isoflavones daidzein and genistein. International Food Research Journal. 2017; 24(5):2140-2141.

Garg C. Khurana P, Garg M. Molecular Mechanisms of Skin Photoaging and Plant Inhibitors. International Journal of Green Pharmacy. 2017; 11(2):219-220.

Farnakope Herbal Indonesia. Jakarta: National Health Ministry; 2013.

Widowati et al. Antioxidant and Anti Aging Assays of Oryza Sativa Extracts, Vanillin and Coumaric Acid. Journal of Natural Remedies. 2016; 16(3):89-90.

Widowati et al. Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and its Compounds. Journal of Reports in Pharmaceutical Sciences. 2018; 23(3):272-273.

Tu PTB, Tawata S. Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia zerumbet. Molecules. 2015; 20:16727-16728.

Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfood DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants. 2017; 6:2-4.

Al-Kawaz HS, Al-Mashhady LAM. Evaluation of The Phytochemical Constituents and Oxidant-Amtioxidant Status for Actinidia deliciosa Extracts. International Journal of Pharmacy & Therapeutics. 2016;7(1):35-36.

Fidrianny I, Elvianna D, Ruslan K. In Vitro Antioxidant Activities in Various Beans Extracts of Five Legumes from West of Java-Indonesia Using DPPH and ABTS Methods. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(3):471-474.

Kladna A. Berczynski P, Kruk I, Piechowska T, Aboul-Enein HY. Studies on the antioxidant properties of some phytoestrogens.Luminescence. 2016; 31(6).

Lai et al. Study of Active Ingredients in Black Soybean Sprouts and Their Safety in Cosmetic Use. Molecules. 2012; 17:11672.

Sitanggang SSDH, Yanti, Lay BW. Effect of Purified Protein Fractions from Soybean on Tyrosinase Inhibition. Sch. Acad. J. Biosci. 2017; 5(8):594.

Budaraga IK, Arnim, Marlinda Y, Bulanin U. Antioxidant Properties of Liquid Smoke Production Variation of Pyrolysis Temperature Raw and Different Concentration. International Journal of PharmTech Research. 2016; 9(6):370.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.


Gedung Kampus 1 Fakultas Kedokteran Universitas Muhammadiyah Sumatera Utara | UMSU

Lantai II,Laboratorium Farmakologi dan Terapi FK UMSU-Jalan Gedung Arca No. 53 Medan-Sumatera Utara ,

Kode Pos 20217 Kontak: 0812-6208-2844,E-mail:

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View My Stats Buletin Farmatera