Effect of Bean Extract (Phaseolus vulgaris L) to LDL and Ox-LDL and Relation of Ox-LDL on LOX-1 Gene Polymorphism 3'UTR
Abstract
Aim: To find out whether bean extract has effects on levels of LDL and Ox-LDL and relation of Ox-LDL on
LOX-1 gene polymorphism 3'UTR188CT.
Method: This research is true randomized experiment with a pretest - posttest design and control group.
Samples were 35 subjects divided into 4 groups. Laboratory experiments using bean extract test affects LDL and
plasma levels of Ox-LDL by Enzyme -linked Immunosorbent Assay (ELISA), test LOX-1 gene polymorphism
3'UTR188CT with restriction enzyme. To evaluate effect of bean extract to LDL and Ox-LDL level were
compared with unpaired t-test. To evaluate difference of Ox-LDL level on LOX-1 gene polymorphism
3'UTR188CT using ANOVA. Results: There was a decrease of LDL found in all treatment groups and elevated
levels of Ox-LDL. TT genotype had higher levels of plasma Ox-LDL both before (49,9 8,3 vs. CC 39.9 19.4
and CT 42,8 61,2,p .648 ) and after treatment (60.3 5.7 vs. CC 55.93 12.6 and CT 53.9 10.3, p .630) than
other genotype.Conclusion: Bean extract can lower LDL cholesterol although it can also increased plasma level
of Ox-LDL. TT genotype had higher levels of plasma Ox-LDL than other genotypes. Require further study to
other antioxidants may have a beneficial effect by lowering the levels of Ox-LDL.
LOX-1 gene polymorphism 3'UTR188CT.
Method: This research is true randomized experiment with a pretest - posttest design and control group.
Samples were 35 subjects divided into 4 groups. Laboratory experiments using bean extract test affects LDL and
plasma levels of Ox-LDL by Enzyme -linked Immunosorbent Assay (ELISA), test LOX-1 gene polymorphism
3'UTR188CT with restriction enzyme. To evaluate effect of bean extract to LDL and Ox-LDL level were
compared with unpaired t-test. To evaluate difference of Ox-LDL level on LOX-1 gene polymorphism
3'UTR188CT using ANOVA. Results: There was a decrease of LDL found in all treatment groups and elevated
levels of Ox-LDL. TT genotype had higher levels of plasma Ox-LDL both before (49,9 8,3 vs. CC 39.9 19.4
and CT 42,8 61,2,p .648 ) and after treatment (60.3 5.7 vs. CC 55.93 12.6 and CT 53.9 10.3, p .630) than
other genotype.Conclusion: Bean extract can lower LDL cholesterol although it can also increased plasma level
of Ox-LDL. TT genotype had higher levels of plasma Ox-LDL than other genotypes. Require further study to
other antioxidants may have a beneficial effect by lowering the levels of Ox-LDL.
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