Profile and Bioactivity of Bioactive Compounds of Beauveria bassiana Fungi Entomopathogens of Endophytes as Plant Growth Boosters

Yusniwati Yusniwati, Trizelia Trizelia, Nurbailis Nurbailis, Magdalena Saragih Saragih

Abstract


The purpose of this study was to identify bioactive compounds of Beauveria bassiana  entomopathogenic fungi derived from insect isolates of Leptocorisa oratorius and endophytic fungi B. bassiana from cocoa plants that have bioactivity as growth boosters.  The research was conducted in the laboratory of Biological Control, Faculty of Agriculture, Andalas University. The study was conducted for 4 months, using the GCMS method. From the results of the study, bioactive compounds identified as growth boosters of B. bassiana endofyt fungi from cocoa plants are compounds Acetic acid, Ethanoic acid, Ethylic acid, Glacial acetic acid, CH3COOH;  n- Hexadecanoic acid Hexadecanoic acid, Palmitic acid;  9-Octadecenoic acid (2)-(CAS), Oleic acid, Red oil, Oelsaure; octadecanoic acid (CAS) Stearic acid, n-Octadecanoicacid, vanilla; Ergosta-5,7,22-trien-3-ol, (3.,beta.,22 E) – (CAS), Ergosterol (CAS);  while  from the  entomopathogenic B. bassiana fungus from L. oratorius insects  are Acetic acid (CAS), Ethylic acid, vinegar acid, Ethanoic acid, CH3COOH, Dianhydromannitol; Isosorbide, D-Glucitol,1,4-3,6-dianhydro-(+)-D-Isosorbide Devicoran;  Hexadecanoic acid, methyl ester (CAS), Methyl palmitate, Uniphat A60;  n-Hexadecanoic acid Hexadecanoic acid, n- Hexadecanoic acid, Palmitic acid; 9-octadecenoic acid, methyl ester, (E)-elaidic acid; 9-octadecanoic acid (Z)-(CAS) , oleic acid, Red oil, Oelsauere; octadecanoic acid, stearic acid, -n-octadecanoic acid, Humko Industrene R; Ergosta-5,7,22-trien-3-ol, (3.beta.,22E)-CAS, Ergosterol (CAS)

Keywords


Bioactivity, B.bassiana fungi, endophyte, entomopathogen.

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References


Affandi,A., Widjayanti,T., Emi, A.A.L., Tamo, H., Afiyanti, M., Handoko, R.N.S.,2019. Endophytic fungi Beauveria bassiana Balsamo accelerates growth of common bean (Phaseolus vulgaris L.). Biol.Technol. Agric.6, 11.

Akello, J., Dubois, T., Coyne, D., Kyamanywa, S., 2008. Endophytic Beauveria bassiana in banana (Balsamo) Vuillemin as an endophyte in tissue culture banana (Musa spp.). J. sInvertebr. Pathol. 96, 34-42.

Charman, L. & Verma, L.R. 2006. Use of certain bio-productsfor insect- pest control. Indian Journal of Traditional Knowledge, 5 (1), 79-82

De-Fatima, A., Modolo, L.V., Conegero, L.S., Pilli, R.A., Ferreira, C.V.,Kohn. L.K., de-Carvalho, J.E. 2006. Lactones and their derrivatives; biological activities, mechanisms of action potential leads fordrug design. Curr.med.chem. 13, 3371-33

Jaber, L.R., Araj, S.E. , 2018. Interactions among endophytic fungal entomopathogens (Ascomycota: Hypocreales), the green peach aphid Myzus persicae Sulzer (Homoptera : Aphididae), and the aphid endoparasitoid Aphidius colemani Viereck (Hymenoptera: Braconidae) Biol. Control 116, 53-61.

Jaber, L.R., Enkerli, J., 2017. Fungal entomopathogens as endophytes: can they promote plant growth? Biocontrol Sci. Technol. 24, 1096-1109.

Jaber, L.R., Vidal, S. 2010. Fungal endophyte ngative effect on herbivory are enhancd on intact plants and maintained in a subsequent generation. Ecol. Entomol. 35, 25-36.s

Jhonson, M., Yamunadevi, M and Gnaraj, W.E 2011. Chromatographic fingerprint analysis steroid in Aerva lanata L. By HPTLC technique. Asian pac J of trop Biomed. 428-433.

Kaushik , J.C., sanjay a., Tripathi N.N., Arya S. 2002. Antifungal properties of some plant extracts against damping off fungi of forest nurseries. Indian Journal of Forestry, 25, 359-361.

Khan I., Anupama, Ssingh, B. 2015. 1,4-Benzodiazepine: An Overview of Biological Properties. Sci.Revs. Chem. Commun, 5(1), 13-20.

Liang – Dong G, Guo – Rui h, Yu w (2008). Seasonal and tissue age influences on endophytic fungi of Pinustabulaeformis (Pinaceae0 in the Dongling Mountains, Beijing. J.IntegraBiol 50: 997-1003

Marston, A. 2007. Role of advances in chromatographic techniques in phytochemistry. Phytochemistry, 68, 2785-2797. s

Posada F. , Aime, M.C., Peterson, S.W., rehner, S.A., Vea, F.E.,2007. Inoculation of coffee plants with the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales). Mycol res 111, 748-757.

Qayyum, M.A., Wakil, W., Arif., M.J., Sahi, S.T., Dunlap, C.A., 2015. Infection of Helicoverpa armigera by endophytic Beauveria bassiana colonizing tomato plants. Biol. Control 90, 200-207.

Qayyum, M.A., Wakil, W., Arif., M.J., Sahi, S.T., Dunlap, C.A., 2015. Infection of Helicoverpa armigera by endophytic Beauveria bassiana colonizing tomato plants. Biol. Control 90, 200-207.

Radji M. 2005. The role of biotechnology and endophytic microbes in the development of herbal friends. Pharmaceutical science magazine 2(3) : 113-126.

Radji M. 2005. The role of biotechnology and endophytic microbes in the development of herbal friends. Pharmaceutical science magazine 2(3) : 113-126.

Ringbom, T, Huss, U, Stenholm, A, Flock, S, Skatteboel, P, Perera, P & Bohlin, L, 2001. Cox-2 Inhibitory Effects on Naturally Occuring and Modified Fatty Acid. J. Nat Prod, vol. 64 pp. 745-749.

Rodriguez RZ, White J.R., J.F., Arnold, A.E, Redman A.S, 2009. Fungal endophytes: diversity and functional roles. New Phytol. 182, 314-330

Russo ML, Scorsetti AC, Vianna MF, Cabello M, Ferreri N, Pelizza S. (2019). Endophytic Effects of Beauveria bassiana on Corn (Zea mays) and Its Herbivore, Rachiplusia nu (Lepidoptera: Noctuidae). Insects 2019, 10, 110; doi: 10.3390/insects 10040110

Saragih M., Trizelia., Nurbailis., Yusniwati. 2018. Endophytic Colonization and Plant Growth Promoting Effect by Entomopathogenic fungus, Beauveria bassiana to red chili (Capsicum annuum L.) with different Inoculation Methods. IOP Conference Series: Earth and Enviromental Science. 305 012070. 7 pp.

Saragih M., Trizelia., Nurbailis., Yusniwati. 2018. Endophytic Colonization and Plant Growth Promoting Effect by Entomopathogenic fungus, Beauveria bassiana to red chili (Capsicum annuum L.) with different Inoculation Methods. IOP Conference Series: Earth and Enviromental Science. 305 012070. 7 pp.

Suryanarayanan TS, Thirunavukkarasu N, Govindarajulu MB, Sase F, Jansen R, Murali TS. 2009. Fungal endophytes and bioprospecting. Fungal biology reviews. 23(1-2):919.DOI:10.1016/j. fbr. 2009. 07. 001.

Suryanarayanan TS, Thirunavukkarasu N, Govindarajulu MB, Sase F, Jansen R, Murali TS. 2009. Fungal endophytes and bioprospecting. Fungal biology reviews. 23(1-2):919.DOI:10.1016/j. fbr. 2009. 07. 001.

Van Bael SA, Maynard Z, Rojas E, Mejia LC, Kyllo DA, Herre EA. 2005. Emerging perspectives on the ecological roles of endophytic fungi in tropical plants.p. 181 -191. In: The fungal Community its Organisation and Role in the Ecosystem (J. Dighton, JF. White, P. Oudemans, eds.). Taylor & Francis, Abington, USA, 597 pp

Van Bael SA, Maynard Z, Rojas E, Mejia LC, Kyllo DA, Herre EA. 2005. Emerging perspectives on the ecological roles of endophytic fungi in tropical plants.p. 181 -191. In: The fungal Community its Organisation and Role in the Ecosystem (J. Dighton, JF. White, P. Oudemans, eds.). Taylor & Francis, Abington, USA, 597 pp

Widyati E. 2017. Understand plant-soil communication in the Rhizosphere area for land management optimization. 10 p.

Widyati E. 2017. Understand plant-soil communication in the Rhizosphere area for land management optimization. 10 p.




DOI: https://doi.org/10.30596/agrium.v26i1.14364

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