Efektivitas Supernatan Isolat Bakteri RNc19 dan RNc43 sebagai Antagonis terhadap Patogen Hawar Malai Padi Burkholderia glumae in Vitro
Abstract
Supernatan dari kultur cair isolat bakteri antagonis diperkirakan mengandung senyawa metabolit yang berfungsi sebagai antimikroba. Kemampuan sebagai antibakteri ditunjukkan oleh sel isolat RNc19 dan RNc43 terhadap Burkholderia glumae, patogen penyebab penyakit hawar malai bakteri pada tanaman padi. Penelitian bertujuan menguji kemampuan antibakteri dari supernatan yang dihasilkan isolat RNc19 dan RNc43 terhadap B. glumae. Isolat bakteri yang digunakan merupakan hasil isolasi rhizosfer Nepenthes clipeata dari Bukit Kelam, Sintang. Produksi supernatan kedua isolat antagonis melalui co-culture, menumbukan isolat antagonis yang ditambahkan dengan B. glumae pada hari ke 2 masa inkubasi. Pengujian sebagai antibakteri meliputi kemampuan daya hambat menggunakan metode difusi cakram agar, menentukan minimum inhibition concentration (MIC) dan minimum bactericidal concentration (MBC). Hasil pengujian menunjukkan supernatan co-culture RNc19 lebih efektif menghambat pertumbuhan bakteri B. glumae dengan daya hambat 77.46%, sedangkan supernatan co-culture RNc43 menunjukkan daya hambat sebesar 46.94%. Kedua supernatan memiliki nilai MIC sebesar 50% dan tidak punya kemampuan membunuh sel B. glumae Hasil penelitian diharapkan dapat menjadi alternatif bahan yang dapat dimanfaatkan sebagai antimikroba patogen tanaman dan pengembangan produksi metabolit sekunder antimikroba.
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DOI: https://doi.org/10.30596/agrium.v27i2.16651
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