(Peer Review, Prosiding) Role of Soluble Heat Shock Proteins 70 in Severity of Dengue Infection

Dr. dr. Nurfadly, MKT.

Abstract


Objective: to prove the role of soluble HSP 70 in the severity of dengue infection by comparing the level of solubI<fiSP 70 in patients who diagnosed as dengue fever (OF), deng!:]e hemon-ha ic fever (DJ- and I healthy people.

Methods: Thi was a pro pective and longitudinal study, samples were taken by using consecutive sampling with a sample size were 22 OF patients, 20 DHF patients and 20 healthy people as a control group. Soluble HSP 70 in sertUn was measured by an enzyme-linked inununosorbent assay (ELISA).

Results: The soluble I-ISP 70 was significantly increased (p=0.003) in DHF patients (0.33 0.17 ng/ml) compared to in OF patients (0.149 0.19 ng/ml) and control group (0.147 0.37 ng/mJ), but there was not significantly increased (p=0.241) in OF patients compare within control group.

Cone ion: This result suggesting that oluble HSP 70 may play important roles in the severity of dengue infection.


Keywords


Soluble HSP70 Dengue fever, Dengue he1mnorhagic fever

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References


,j- World Health Organization. Comprehensive Guidelines for Prevention and Control of Dengue and

Dengue HaemoIThagic Fever. 2011

. World Health Organization. Dengue: Guidelines for

diag.s, treatment, prevention and control. 201 I

ii' A. Srikiatkhachorn, R.V. Gibbons, S. Green, D.I-I.

-braty, SJ. Thomas, et al. Dengue hemorrhagic fever:

If sensitivi and s ecificity of the , orld health

pnization definition for identification of severe cases off den e in Thailand 1994-2005. Clin Infect Dis, vol. 50,

, pp. 1135-1143.

. G.N. Malavige, N. Fernando, G.Ogg, Pathogenesis of Dengue viral infections. Sri Lankan Joum lnfecti Dis,

vol. I, 201 !,pp. 2-8 OOI: 1016038/sljid vlil2 987

. K.H. Sellahewa, Pathogenesis of Dengue

Haemon-hagic Fever and Its Impacton Case Management.

e

Infclfs Diseases. 2013 DOI: 10.5402/2013/571646

(6). M.P. Mayer and B. Bukau. Hsp70 chaperones:

Cellular functions and molecular mechanism. Cell. Mol.Life Sci, vol. 62, 2005, pp. 570-586. DOI:

IO. I 007 /s000 18-004-4464-6

. F. Amorim and P. Moseley, Heat Shock Protein and

inflamation. Heat Shock Proteins and Whole Body

Physfjogi. Springer Science, 2010, pp 57-88

ij J. Kocsis, B. Madaras, E.K Toth, G. Fust, Z.

lif haszka, Serum level of soluble 70-kD heat shock

rotein is associated will mortali , atients , t

colorectal cancer without distant metastasis. Cell Stress

Id Chaperones, vol. 15, 2010, pp.143-151.

DOII0 1007/s12192-009-0128-7

II]. MA Bausero. R. Gaspar, G. Multhoff and A. Asea,umor recognition: active release of heat shock protein 72.

lijl A Asea, S.K. Kraeft, EA Kurt-Jones, MA irenson, L.B. Chen, RW. Finber , et al. HSP 70 stimulates c tokine production throu ha CDl4-dependen

ljhway, demontrating its dual role as achaperone and cytokaNat Med, vol.6, 2000, pp. 435-42

[I I].

Ather.erosis, vol. 185, 2006, pp.32-38

. MH. Manjili J. Park J.G. Facciponte and J.R

jeck. HSP 110 induces "danger signal" upon interaction with antigen presenting cells and mouse mammaty carcinoma, Imunolagy, vol. 210, 2005, pp.295- 303

. LA Espada-Mmao and K.Morita, Dengue and Soluble Mediators of the Innate lmmtme System, Tropical Medicine and Health Vol. 39 No.4, 201 I, pp. 53-62

DOI:,2149/tmh201 l-S06

. Z. Kou, M Quinn, H. Chen, W. W. Rodrigo, RC. Rose J.J Schlesin er X. Jin. Monocytes but not T or B cells

[ 15] J I-I.I-I. Williams and C Hunter-Lavin,"Roles of extracellular heat shock proteins: a new sense of dan

YWang, T. Whittall, E. McGowan, J Younson, C. Kelly, L.A. Bergmeier, M. Singh, and T. Leimer. Identification of stinmlating and inhibitory epitopes within the heat shock protein 70 molecule that modulate cytokine production and maturation of dendritic cells. J

Imn.l. Vol. 174, 2005, pp. 3306-16.

lijl H.J.Zhang,_L. Xu, V.J. Drake, L. Xie , L.W. Oberley C. Kre el Heat-induced liver injw in old rats isfjsociated with exaggerated oxidative stress and altered transcription factor activation. FASEB J Vol. 17 (15),

.2293-5.

L...Io, LL Wang, MF. Shaio, C.L Kao, D.M

rnng, JI-I. Lai. Infection of human dendritic cells by dengue virus causes cell matmation and cytokine

prod.on. J lnunw1ol, vol. 166, 2001, pp.1499- 1506.

[ 19] J. Cam isi T.I-I. Leem M. Fleshner Stress-induced

ltracellular HSP72 is a functionally significant danger signal to the immune system Cell Stress Chaperones .

Fall, vol. 8(3), 2003, pp.272-86.


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